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  Zanko J Med Sci:  Dec. 2016; 20 (3): 1513-1519

Correlation between Endothelin-1 and oxidative stress in apparently healthy obese men

Nazlin Shakir Malla Ahmed & Sherwan Rahman Sulaiman


Background and objective: Obesity is the risk for many disease conditions and is becoming a global issue. Endothelial dysfunction and oxidative stress are linked to obesity. Endothelin-1 is a useful marker of endothelial dysfunction. Oxidative stress can be assessed by estimation of lipid peroxidation byproduct malondialdehyde. In this study, we tried to find out the correlation between Endothelin-1 and oxidative stress among apparently healthy obese men. 

Methods: Eighty apparently healthy non-smoker adult men have participated in this study. Their age ranged between 18 and 50 years. Their serum Endothelin-1 (ELISA), malondialdehyde and lipid parameters (biochemical assays) have been estimated.

Results: Subjects were divided into three groups: normal weight (n = 25), overweight (n = 34) and obese (n = 26) basing on their body mass index. The study shows that Endothelin-1 is significantly correlated with each of Malondialdehyde and body mass index (r = 0.262, P = 0.019; r = 0.65, P <0.001 respectively). Malondialdehyde is also significantly positively correlated with BMI (r = 0.378, P = 0.001).

Conclusion: Endothelial dysfunction and oxidative stress coexist in obesity. But which of them precedes the other? Is still a question needs to be further studied

Keywords: Obesity; BMI; Endothelin-1; Malondialdehyde.


1. Obesity and overweight. World Health Organization (WHO); 2012. Fact Sheet No. 311. (Accessed February 7, 2014), available from :

2. Debra A , Krummel. Medical nutrition therapy for cardiovascular disease. Krause, editor. Krause’s Food, Nutrition and Diet Therapy. 12th ed. Philadelphia, USA: Saunders; 2008. PP 833-64.

3. Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y, et al. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 1988; 332: 411-5.

4. Davignon J, Ganz P . Role of Endothelial Dysfunction in Atherosclerosis. Circ J 2004; 109 (3): 27-32.

5. Mather KJ, Mirzamohammadi B, Lteif A, Steinberg HO, Baron AD. Endothelin contributes to basal vascular tone and endothelial dysfunction in human obesity and type 2 diabetes. Diabetes 2002; 51:3517-23.

6. Amirkhizi F, Siassi F, Minaie S, Djalali M, Rahimi A, Chamari M. Is obesity associated with increased plasma lipid Peroxidation and oxidative stress in women? ARYA Atheroscler 2006; 2 (4): 189-94.

7. Vincent HK,Taylor AG. Biomarkers and potential mechanisms of obesity-induced oxidant stress in humans. Int J Obes (Lond) 2006; 30: 400-18.

8. Karatas F, Karatepe M, Baysar A. Determination of free malondialdehyde in human serum by high-performance liquid chromatography. Anal Biochem 2002; 311: 76-9.

9. Adly AM . Oxidative stress and disease: An updated review. Res J Immunol 2010; 3 (2): 129-45.

10. Wei M, Gaskill SP, Haffner SM, Stern MP. Waist Circumference as the Best Predictor of Noninsulin Dependent Diabetes Mellitus (NIDDM) Compared to Body Mass Index, Waist/hip Ratio and Other Anthropometric Measurements in Mexican Americans- A 7-Year Prospective Study. Obes Res 1997; 5(1): 16-23.

11. Waist circumference and waist–hip ratio: report of a WHO expert consultation. World health organization, 2008. Geneva. (Accessed on February 8, 2014). Available from http: //

12. Amanullah M, Zaman GS, Rahman J, Rahman SS . Lipid peroxidation and the levels of antioxidant enzymes in hypertension. Free Radicals and Antioxidants 2012; 2 (2): 12-8. 80

13. Gutteridge J, Halliwell B . The measurement and mechanism of lipid peroxidation in biological systems. Trends Biochem Sci 1990; 15: 129-35.

14. Caballero AE . Endothelial Dysfunction in Obesity and Insulin Resistance: A Road to Diabetes and Heart Disease. Obes Res 2003; 11: 1278-89.

15. Maeda S, Jesmin S, Iemitsu M, Otsuki T, Matsuo T, Ohkawara K. Weight Loss Reduces Plasma Endothelin-1 Concentration in Obese Men. EBM 2006; 231: 1044-7.

16. Lilyasari O, Kosasih A, Liastuti L, Librantoro, Gunawan I R, Idham I, et al. Increased Circulating Endothelin-1 in Obesity-related hypertension, but not in Obesity or Hypertension Alone. Circ J 2007; 116 (2): 341-4.

17. Cerri E. Obesity and endothelial dysfunction: mechanisms, method development and interventions. PhD thesis. University of Birmingham Institutional Research Archive. eTheses Repository; 2009. [Online] [Accessed on 14th, Oct, 2012]. Available from:

18. Selvakumar C, Maheshwari U, Suganthi, Archana . Oxidant-Antioxidant disturbance in men classified as obese according to the preliminary WHO guidelines for Asians. JSPB 2012; 8 (1): 172-81.

19. Lachieitner M, Koch T, Harold M, Dzien A, Hoppiahler F. Tumor necrosis factor-alpha plasma level in patients with type 1 diabetes mellitus and its association with glycaemic control and cardiovascular risk factors. J Intern Med 2000; 248: 67-76.

20. El-Mesallamy H, Suwailem S, Hamdy N. Evaluation of C-Reactive Protein, Endothelin-1, Adhesion Molecule(s), and Lipids as Inflammatory Markers in Type 2 Diabetes Mellitus Patients. Mediators Inflamm; 2007.(Accessed on 28th, Aug, 2012), Available from:

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